Publish Time: 2024-09-02 Origin: Site
During the process of cell cryopreservation, some experimenters choose to wrap the mouth of the cryopreservation tube with sealing film to enhance the sealing. However, this is actually a common misunderstanding.
The sealing film is not suitable for ultra-low temperature environments, especially liquid nitrogen environments with temperatures as low as -196°C. Under such extreme conditions, the sealing film is prone to cold shrinkage and embrittlement, causing fragments to fall off. Not only does it fail to protect, it may also cause contamination problems.
Why is it not recommended to use parafilm for cell cryopreservation?
· Risk of material denaturation
· Operational interference
·Sealing film size problem
· Save money and time
· Space issues
Summary of experimental experience
Years of practice have shown that cryopreservation tubes without a sealing film can also ensure the sealing of the cryopreservation solution without increasing the risk of contamination or affecting the safety of the cryopreservation tube.
Tips for using cryovials
Before placing the cryovials into the programmed cooling box, it is recommended to lightly tighten the caps. This can prevent liquid nitrogen from entering the tube due to the slight shrinkage of the gap in the tube cover after cryopreservation, thereby reducing the risk of bloating and bursting during cell recovery.
Aijin Cryotube
At the same time, choosing the right cryovial is also crucial. Aijin cryopreservation tubes are made of imported medical-grade polypropylene (PP) and can be used for high-temperature and high-pressure sterilization. They can be used stably in the temperature range of -196°C to 121°C, ensuring the safety of cell cryopreservation. In addition, Aijin cryovials are pyrogen-free, endotoxin-free, DNase/RNase-free, and have been sterilized by electron beam, which can effectively ensure the reliability of experimental results.
Aijin cryopreservation tubes are available in a variety of capacity specifications, and the boxes are in compliance with SBS standards, which can meet different experimental needs. The product production environment is strictly controlled in a 100,000-level dust-free workshop to ensure product consistency and stability. In general, choosing the right cryogenic tube can not only effectively protect your samples, but also make your experimental operations more efficient and safer.