Publish Time: 2024-06-03 Origin: Site
As a commonly used cell culture consumable, triangular culture shake flask has a unique conical design and is mainly used in cell and bacterial culture, media configuration, reagent storage, heating, cooling, etc.
Advantages of PC material triangular culture shake flask
Triangular culture shake flask is a type of cell culture product based on Erlenmeyer flasks. Compared with glass Erlenmeyer flasks, PE has the following advantages:
1) Not easily broken;
2) The breathable cover is sealed and easy to take after heating;
3) Use liquid-proof materials to protect precious samples;
4) Clear scale and transparent bottle material make it easy to directly observe the situation inside the bottle during culture;
5) For large-capacity triangular shake bottles, there is an anti-slip design at the mouth of the bottle, which is convenient for stable grip and prevents the bottle from being too heavy after being filled with liquid and making it difficult to carry.
The optimal working volume of the triangular shake flask
When the Erlenmeyer flask is used for culture, it is usually in a shaking shaker environment. Too much may cause liquid to splash to the bottle mouth during shaking, increasing the risk of contamination. At the same time, it compresses the gas space in the bottle, which is not conducive to gas exchange during culture. If it is too little, the depth of the medium liquid in the bottle may be insufficient, reducing the three-dimensional growth space of bacteria and cells.
The difference between baffles and flat bottoms
The difference between a baffled and a flat bottom is that there is a raised baffle on the bottom edge. The baffle can increase the shear force on the liquid during the shaking process of the shaker, improve the gas exchange efficiency, thereby increasing the dissolved oxygen. It is suitable for microorganisms or cell culture with large oxygen demand, and the effect is better when cultivating at low rotation speed.
Aijin products-triangular culture shake flask
| Material | The bottle body is made of medical polycarbonate (PC), which has high transparency, strong impact resistance and anti-oxidation |
| Package | Individually sterile packaging |
| purity | No pyrogen, no endotoxin, no DNase/RNase, no cytotoxicity |
| Sterile | Electron beam sterilization, SAL=10-6 |
| production environment | Clean production in a 100,000-level dust-free workshop with small differences between batches |
| Storage conditions | Store dry at room temperature to prevent ultraviolet rays; three-year shelf life (packaged intact) |
| Usage restrictions | The product is recommended for one-time use and can be sterilized by high temperature and pressure at 121°C. |
Design and Optimization
A baffle design is added to the bottom to effectively improve the gas exchange rate;
The scale is clear, making it easy to observe the medium capacity;
There are two options: breathable cover and sealing cover. The breathable cover is covered with a 0.22μm hydrophobic film, which blocks bacteria and is breathable, which facilitates continuous gas exchange and effectively prevents microbial contamination;
The bottom groove design causes the culture liquid in the bottle to form a vortex and increase the amount of dissolved oxygen;
The wide-mouth bottle neck design facilitates sampling and reduces the generation of bubbles during liquid filling;
The bottle neck is designed with an anti-slip mouth for easy and stable grip;
The unique triangular design makes it easier for pipettes or cell scrapers to reach the corners of the bottle, making cell culture operations more convenient.