Publish Time: 2024-11-04 Origin: Site
The growth and quality of cells are often closely related to the culture environment. The cell culture dish is the basis of this environment, such as an exquisite life greenhouse, which provides the necessary space and conditions for the reproduction and development of cells. Therefore, it is crucial to choose the appropriate culture dish, which not only affects the growth status of cells, but is also the key to ensuring the reliability of experimental results.
Aijin provides various types of cell culture dishes to meet various scientific research needs. Next, let us take a look at how to choose a suitable petri dish according to the experiment .
01. Select the specifications according to the required number of cells.
Aijin cell culture dishes are available in three specifications: 35mm, 60mm, and 100mm. The larger the diameter, the larger the cell growth surface area, and the greater the number of cells that can be cultured.
35mm petri dish
Suitable for small-scale cell culture experiments, usually add 3 mL of culture medium, and the amount of cells that can be harvested when the monolayer is full is about 2×10⁶.
60mm petri dish
Suitable for medium-scale cell culture experiments, usually add 5 mL of culture medium, and the amount of cells that can be harvested when the monolayer is full is about 5×10⁶.
100mm petri dish
Suitable for large-scale cell culture experiments, usually add 10mL of culture medium, and the amount of cells that can be harvested when the monolayer is full is about 13.7×10⁶.
Choosing the appropriate size also needs to consider the type of cultured cells . For example, fibroblasts and hepatocytes usually choose larger size culture dishes (such as 60mm or 100mm). For cells that require low-density cell growth (such as neuronal cells and lymphocytes), smaller size culture dishes (35mm) are usually selected.
02. Whether the surface has been treated with TC
The surface treatment method should be selected according to the cell type. A hydrophilic gene is introduced on the surface of the Aijin cell culture dish, which increases the adsorption to cells after TC treatment. It is suitable for the culture of adherent cells, while the untreated surface is suitable for the culture of suspended cells or microorganisms.
03. Is it designed with an easy-to-grip ring?
Our petri dishes are optionally equipped with easy-grip serrated rings, which facilitate the experimenter to grasp the petri dish more stably and reduce the risk of experimental contamination. At the same time, the annular protrusions can be tightly integrated with the bottom when stacked to ensure the stability of stacking.
In short, choosing a suitable cell culture dish requires comprehensive consideration of the purpose of the experiment, the type of cells to be cultured, and the ease of operation to ensure high efficiency of the experiment and reliability of the results.