Publish Time: 2024-11-18 Origin: Site
QUESTION
How to fight the 'defense war' of biological samples?
ANSWER
When freezing samples, dressing them up is key!
Biological samples occupy an important position in medical research, and their quality plays a key role in the accuracy and reliability of clinical results. There are two main ways to preserve biological samples: cryogenic and normal temperature. Different scientific research needs will put forward specific requirements for storage temperature. Correspondingly, there will also be different dressing requirements, which need to be determined according to the specific preservation method. Only in this way can we better 'defend' biological samples.
PART 01 -196℃/-150℃
-196°C is the temperature at which liquid nitrogen evaporates. At this temperature, the physiological and biochemical activities inside the sample almost completely stop, thus enabling long-term stable storage.
However, the sample is completely immersed in liquid nitrogen, and free tissue fragments may bring potential risks of cross-contamination. In order to solve this problem, the gas-phase liquid nitrogen preservation method (-150°C) came into being. This method places the sample in a gas-phase liquid nitrogen environment above liquid nitrogen, effectively reducing the possibility of cross-contamination. In addition, electric refrigerators can also reach low temperature storage of -150°C to meet long-term storage needs.
In this temperature range, plastic containers are severely tested, and many disposable plastic consumables are prone to embrittlement and cracking.
Therefore, it is recommended to use Aijin cryopreservation tubes, which are made of PP material treated with special low-temperature resistance technology. Even in -196°C liquid nitrogen, they can maintain a low tube burst rate and leakage rate, thereby ensuring the safety of the sample.
PART 02 -80℃/-20℃
-80°C is a commonly used sample storage temperature, especially suitable for preserving the activity of biological macromolecules. -80°C cryopreservation is an ideal sample preservation method for frozen nucleic acids, which can be maintained for several years or longer. However, RNA is susceptible to degradation by RNases that are widely distributed in cells and various tissues.
The storage time of RNA in a stabilizer generally does not exceed 5 years. Therefore, in order to preserve RNA activity for a long time, it is recommended to use a lower temperature, or to use a small part of the sample to extract RNA and store it simultaneously with the remaining samples.
At -80°C, Aijin's HDPE reagent bottles, screw-top tubes, cryopreservation tubes, centrifuge tubes and other products all show excellent anti-freeze properties and are suitable for storing reagents or biological samples.
At the same time, the preservation of proteins also mainly relies on deep cryopreservation, and short-term storage can also be carried out in a low-temperature refrigerator at -20°C. Under this condition, Aijin deep well plates and PCR series consumables are also suitable for the storage and use of samples in a -20°C environment.
PART 03 stored at room temperature
In view of the inconveniences in transportation and operation of deep cryogenic freezing, normal temperature preservation methods have gradually attracted attention. This method uses special technology or containers to place dehydrated and dried samples in a closed environment, or add protective agents to the samples to inactivate related enzymes, thereby achieving the effect of protecting the samples.
Under normal temperature conditions, the selection of containers should pay attention to their sealing and chemical resistance. Aijin screw-top tubes and centrifuge tubes made of PP material are better choices and can effectively protect samples.
By selecting reasonable temperatures and consumables, it can not only extend the storage stability of biological samples, but also provide reliable protection for medical research. Choose Aijin, choose quality, and make the storage of your biological samples safer and more worry-free.