Publish Time: 2024-01-26 Origin: Site
The enzyme plate is an indispensable experimental tool in the Enzyme Linked Immunosorbent Assay (ELISA). It plays a key role in the determination of the purity, concentration and ratio of antigens, antibodies, labeled antibodies or antigens in immunological reactions; buffer type, concentration and ionic strength, pH value, reaction temperature, time and other conditions.
Aijin Bio enzyme plate
● High-quality imported polystyrene (PS) material;
● ANSI/SBS international standard, the size is accurately adapted to automated instruments;
● The adsorption effect of each hole is uniform and stable, and the error between holes is small;
● The transparent plate has high light transmittance, CV value <5%, and more sensitive measurement;
● The bottom is flat and uniform, reducing the gap between holes and ensuring the accuracy of the experiment;
● There are clear numbers and letters on the edge of the hole to facilitate sample marking;
● 1536 holes has an increased number of holes compared to 96/384 holes, and a very small amount of sample can be loaded to save detection costs.
Frequently Asked Questions about ELISA Plate
Question: How to choose the color of the enzyme plate?
Answer: Transparent enzyme plate is the most commonly used plate, suitable for light absorption detection (colorimetric analysis) of color reaction, cell culture and observation.
The white enzyme plate can block light from interfering with the transmission between wells, and can enhance signal reflection and enhance light signal intensity, making it suitable for chemiluminescence detection.
The black enzyme plate itself will absorb light, so its signal is weaker than the white enzyme plate. It is generally used to detect stronger light, such as fluorescence reactions.
Q: What are the differences in application of 96/384/1536 holes?
Answer: These three types of microplates are suitable for automated equipment. The 96/384-well plates can also be used with pipettes for manual pipetting, but the 384-well manual pipetting operation is more cumbersome.
Compared with the 96-well detection plate, the 384-well plate has 6 times the number of holes, which greatly improves the detection efficiency. At the same time, the amount of sample loaded in a single well is reduced, saving reagents. The square hole design saves space on the entire board and maximizes the utilization of single hole capacity space.
Compared with 384 holes, the number of 1536 holes is increased by four times, and a very small amount of sample can be loaded to save costs to the greatest extent. The SBS specifications perfectly match the automated equipment, and the positions of each hole strictly correspond to the suction head.
In application, the selection is mainly based on the experimental throughput, whether the machine supports it, and the liquid volume. If the number of samples is small or the volume of liquid added is large, you can choose a 96-well plate. Otherwise, you can consider a 384/1536-well plate. Before choosing, you can first confirm the type of microplate plate supported by the microplate reader.
Q: What are the differences in application of different bottom types?
Answer: The refractive index of flat-bottomed microplates is low and is suitable for detection with microplate readers.
The U-bottom microplate has a high refractive index and is convenient for operations such as adding, aspirating, and mixing. It can be used to observe color changes directly without placing it on the microplate reader.
V-bottom microplates are generally used for cell killing experiments and can bring effector target cells into close contact.
Q: What is the difference between high/medium binding strength?
Answer: After the surface of the high-binding enzyme plate is treated, the protein binding capacity increases and can reach 400~500ng/cm². The use of this type of microplate can improve the sensitivity and relatively reduce the concentration and dosage of coating proteins. The disadvantage is that non-specific reactions are more likely to occur. After the antigen or antibody is coated, non-ionic detergents cannot effectively block the parts that are not bound to the protein, so protein must be used as a blocking agent.
The medium-binding force enzyme plate passively binds to proteins through surface hydrophobic bonds and is suitable as a solid-phase carrier for macromolecular proteins with a molecular weight >20kD. Its protein-binding capacity is 200~300ng/cm². Suitable as a solid phase carrier for unpurified antibodies or antigens to reduce potential non-specific cross-reactions. This type of plate can be used as a blocking solution with inert protein or non-ionic detergent.
Ordering information
96 holes:
384 holes:
1536 holes: