Publish Time: 2026-04-29 Origin: Site
PCR consumables are the core carrier to ensure amplification efficiency and result reliability. Their reasonable design will directly affect the uniformity of heat conduction, signal accuracy, and automation adaptability.
Key factors such as skirt design, color, volume, and material need to be comprehensively considered when selecting to match different experimental throughputs, instrument types, and sample management needs. It is widely used in #molecularbiology 、#biochemistry, and #pharmaceutical research and development, and other fields.
Researchers can choose different specifications of #PCR consumables based on different throughput requirements and instrument compatibility: PCR plates are suitable for high-throughput amplification, eight-tube strips are suitable for medium-throughput experiments that require flexible combination, and single tubes are mostly used for low-throughput or special reaction conditions.
Part1
Skirt design adapts to experimental scenarios and automation needs
Choosing the right #PCR plate is crucial to the experiment. Its thermal conductivity efficiency, temperature uniformity between wells and sealing directly affect data accuracy and result reproducibility.
As the core feature of the PCR plate, the skirt design determines its compatibility with the instrument module. This compatibility is the key to achieving efficient heat conduction, preventing sample leakage, and further ensuring compatibility with automated equipment, reliable sealing, and consistency of experimental results.
Skirtless PCR plate
The edgeless panel is compatible with most PCR machines and qPCR machines, and the design is easy to cut. However, the pipetting stability is low, and it needs to be used with a plate holder, so it is not suitable for automated applications.
Half-skirted PCR plate
Taking into account automation compatibility and manual operation convenience, the edge panel is short, providing stable support for robotic arm grabbing, and can well meet the automation needs of medium-throughput experiments.
The half-skirted PCR plates provided by A-gen Biotech support detachable models in different colors (red/yellow/blue/green). Sample batches can be quickly distinguished through color marking to facilitate sample classification management in high-throughput experiments.
Full skirt PCR plate
It has an edge panel that completely covers the height of the board, which significantly improves the structural rigidity of the board. It is suitable for automated experimental applications and is easy for mechanical grippers to grasp.
In addition, #A-gen biotics also provides high-skirt PCR plates, which can meet the needs of a variety of experimental scenarios and provide flexible and stable support for experiments with different throughputs and specifications.
Part2
Color selection adapted to experiment type and sample management
In ordinary PCR experiments, transparent or white reaction tubes are usually used.
In qPCR experiments, white reaction tubes can significantly improve fluorescence collection efficiency and signal-to-noise ratio due to their high reflection and low light transmission characteristics, and can reduce signal crosstalk between wells and improve repeatability. However, this type of reaction tube is not suitable for instruments with bottom or side lighting.
Select suggestions
Transparent tubes are used for ordinary PCR; white tubes are used for qPCR (non-bottom/side lighting instruments); multi-batch sample management is equipped with five-color transparent eight-strip tubes with caps.
In addition, we also provide eight-cap tube strips in five transparent colors (transparent red/transparent yellow/transparent blue/transparent green/transparent purple), which facilitates experimenters to quickly distinguish and systematically manage samples, and optimize the experimental process.
Part3
Volumetric specifications balance instrument fit and reaction efficiency
A-gen Bio's 96-well PCR plates and eight-tube strips include two specifications: 0.2mL (high volume) and 0.1mL (low volume). The standard volume of the 384-well plate is 40μL, and the single tube is available in 0.2mL specifications.
The left is 0.1mL specification, the right is 0.2mL specification
high volume
Compatible with most thermal cyclers, qPCR machines, and sequencers.
low volume
This minimizes the space above the reaction system, reduces the impact of evaporation, and improves heat transfer efficiency.
Therefore, under the premise of meeting the requirements of instrument adaptation, low volume is recommended first.
Part4
Material guarantee ensures reaction purity and stability
A-gen Bio's PCR consumables are made of imported medical-grade polypropylene (PP) material, which has excellent thermal stability and can withstand repeated high and low temperature cycles without being easily deformed.
The material itself has no human DNA residue, does not contain PCR inhibitors and DNase/RNase, effectively avoiding external interference, ensuring reaction purity and amplification efficiency, and ensuring accurate and reliable experimental results.
Part5
Choose A-gen, choose quality
A-gen Bio's PCR consumables improve reaction efficiency through precise skirt design, scientific color scheme, optimized sample management, low-volume specifications, and medical-grade PP material to ensure the purity of results. It provides a stable, efficient, and reliable one-stop solution for different throughputs and experimental scenarios, helping scientific researchers achieve more efficient and accurate experimental results.