Publish Time: 2024-03-25 Origin: Site
Cell experiment is an extremely important part of the life science discipline, which greatly promotes the development of the biomedical industry. It leads to a dazzling array of equipment, reagents, and consumables. There are many types of cell culture containers alone. This article will give an overview of cell culture products for your reference and study.
cell culture container
Cell culture dish: Large opening, easy to operate, suitable for use when extracting and culturing primary cells, and convenient for placing tissue fragments, etc. However, there is no corresponding anti-contamination function, so you need to pay special attention to aseptic operation when conducting cell-related experiments.
Cell culture bottle: It is the most suitable culture vessel for long-term cell culture, large-scale cell expansion and contamination prevention in the laboratory. The bottle mouth is small and equipped with a sealing cap or a breathable cap, which can effectively prevent contamination in the incubator and reduce the risk of contamination. However, the small bottle mouth also brings operational inconvenience. When performing cell passaging, it is best to use an extended tip or a serological pipette to reach the bottom of the bottle to absorb the culture medium more safely. There are also triangular shake flasks suitable for suspension cell culture, which have large space and tighten the bottle mouth to prevent contamination.
Cell culture plates: There are various models of 6/12/24/48/96/384-well plates. The sizes are all uniform SBS international standard sizes and are suitable for most automated machines on the market. Different throughput well numbers are suitable for various subsequent cell experiments, such as transfection, transwell experiment, scratch experiment, immunofluorescence, HE staining, etc. For multi-throughput cell experiments, different sample groups are set up to facilitate the operation of the experimenters.
Summary: Petri dishes are used for adherent primary cell culture, cell culture flasks are used for passage and expansion of mature cells, triangular shake flasks are used for suspension cell culture, and culture plates are used for cell use.
Cell culture equipment
Cryopreservation products: Cells need to be cryopreserved when they are not being cultured temporarily. Low-temperature freezing can make cells enter a dormant state. The cryopreservation tube can withstand ultra-low temperature refrigerators -80 degrees Celsius and even liquid nitrogen environments -196 degrees Celsius. It is a safe, stable and low-temperature-resistant container that can perfectly protect your cells. Similar to them are screw-top tubes. Their shapes and appearances are very similar, but the materials are different. Screw-top tubes can only withstand low temperatures of -80°C. They are often used for the preservation of small doses of reagents or short-term cell cryopreservation.
Serological pipette: The growth of cells requires nutritional support from the culture medium. For example, a T75 cell culture flask requires 10-20mL of culture medium, and cell culture operations need to be done with caution to prevent contamination. If you use a 1mL tip, you need to repeat the suction and discharge operations dozens of times, which greatly increases the risk of contamination. At this time, a large-capacity and accurately graduated serological pipette became a savior for scientific research. However, its use requires a special-caliber pipette or electric pipette.
Centrifuge tubes: Centrifuge tubes are consumables often used in cell experiments. It is needed for the dispensing of serum, culture medium, and buffer, the transfer of culture medium, and the centrifugation after cell collection.
Cell scraper: Some cells are very stubborn after attaching to the wall. It is difficult to release them from the adherent state by digestion with enzymes alone. This requires a little help from a cell scraper to scrape off the cells through physical external force. However, the cell scraper will damage the cell state to a certain extent and cannot be used casually. Only some cells will need to be used. At the same time, when scraping, scrape gently in the same direction.
Cell culture consumables are the prerequisite for ensuring good cell growth. They need to be comprehensively considered in combination with cell culture methods, culture scale, laboratory conditions and other factors. Only by selecting appropriate and high-quality cell culture consumables can we help us conduct better experiments and ensure the effect of culture.