Sharing useful information | Frozen storage tube burst? What details were not done well?

This situation will not only result in the loss of experimental samples, but may also cause harm to experimental personnel. Therefore, it becomes crucial to understand why cryovials burst and how to avoid this.

In experiments, we often need to freeze cells for long-term storage at a temperature of -130°C or lower. In a liquid nitrogen tank, the temperature of the gaseous layer ranges from -140°C to -180°C, but liquid nitrogen often infiltrates.

The root cause of the cryopreservation tube bursting is that liquid nitrogen remains in the tube. When we take out the cryopreservation tube from the liquid nitrogen tank, the temperature inside the tube rises rapidly, causing the liquid nitrogen to vaporize rapidly and produce a large amount of nitrogen, which increases the pressure inside the tube and eventually causes the tube to burst.

1. Let the liquid nitrogen flow out and then open the cryopreservation tube to perform cell resuscitation operations.

After removing the cryopreservation tube from the liquid nitrogen tank, you need to place the cryopreservation tube horizontally or upside down on the table for tens of seconds as soon as possible. Wait for the liquid nitrogen to flow out before opening the cryopreservation tube to resuscitate the cells.

2. Add freezing solution

When freezing cells, add more cryopreservation solution (for example: add 1.8ml of cryopreservation solution to a 2ml cryopreservation tube) to reduce the gas space and tighten the cryopreservation tube mouth.

3. Use cryopreservation sleeves

It can effectively isolate liquid nitrogen and prevent it from directly contacting the cryopreservation tube and penetrating into the tube.

4. Stored in liquid nitrogen gaseous layer

Storing cryovials in a gaseous layer of liquid nitrogen can effectively prevent liquid nitrogen from penetrating into the cryovials. However, only a small amount of liquid nitrogen can be stored in the liquid nitrogen tank, and liquid nitrogen needs to be added frequently.

In summary, by correctly taking corresponding operating measures, the risk of cryogenic tube bursting can be effectively reduced, the integrity of experimental samples can be protected, and the safety of laboratory work can be ensured. At the same time, during the experiment, we must always remain vigilant, strictly abide by the operating procedures, and pay attention to every detail to ensure the safety of ourselves and others. Let us work together to create a safe and reliable experimental environment and contribute to the progress of scientific research!