Only those who have done WB will know how easy it is for WB to overturn! ! ! 'The longest road in scientific research career is the detour of WB experiment...' I want to share some of my experiences with everyone. #01-Sample Preparation To ensure that the sample protein is protected from degradation, the sample was quickly frozen in a liquid nitrogen environment immediately after collection, and then stored in a -80°C constant temperature freezer. During this process, be sure to configure the corresponding batch of positive control samples to grind the tissue: grind thoroughly, add lysis solution (1:10) according to weight, sonicate for 1 minute, and place on ice for 30 minutes. (After lysis: ① Observe whether the lysis solution is viscous. The reason for viscosity may be that there is no ultrasound, or the amount of lysis solution is not enough, and you need to add lysis solution; ② After centrifugation, observe whether the supernatant is clear and whether there is white precipitate below. If it is large, it is not well lysed.) After weighing the soft tissue, add the lysis solution in proportion and grind it with a handheld grinder.

Gas is one of the necessary conditions for the survival of human cell culture. The required gases mainly include oxygen (O2) and carbon dioxide (CO2). O2 participates in the tricarboxylic acid cycle, producing energy for cell growth and proliferation and synthesizing various components required for cell growth. During in vitro culture, cells are generally placed in a mixed gas environment of 95% air and 5% CO2, so CO2 cannot be used under sealed conditions. Generally, the pH of cell culture medium is between 7.0-7.4.

The large-mouth pipette tip is a pipette tip designed for pipetting difficult-to-transfer and fragile samples. Compared with ordinary suction tips, it has a larger opening at the lower end, making it easier to drain liquids and providing smaller mechanical shear. In terms of application, large-mouth pipette tips are ideal for absorbing viscous materials, genomic DNA, and cell culture fluids. For viscous liquid biological macromolecules and fragile samples, Aijin Biotech's 5mL and 10mL large-capacity pipette tips are available in large and small mouth designs. The suction efficiency of the large-mouth pipette tips when absorbing viscous samples is now tested. Experimental method for testing the suction efficiency of 5/10mL large-mouth tips: Test the functions of 5mL and 10mL large-mouth tips by comparing the efficiency and accuracy of glycerol absorption with large and small mouth tips of the same specifications. Instruments and reagents: electronic balance, glycerin Experiment content: 1. Place the 100mL capacity beaker on the electronic balance, peel it, add 20mL of glycerin to it, weigh and record. 2. Use 5m

The main function of plate consumables is as storage containers and reaction vessels. The sealing film can closely fit the surface of the deep-well plate, so that each well of the deep-well plate can be sealed to prevent evaporation and cross-contamination of the solution. They have different materials and light transmittances, and are widely used in a variety of experiments (such as conventional PCR, qPCR, ELISA, cell culture, long-term storage, automated workstation processing, etc.). Have you encountered any of these situations during use? When doing PCR/qPCR experiments, if the membrane is always unevenly attached, you can use a pressure-sensitive sealing membrane. The initial viscosity is extremely low and only becomes viscous when pressed. It is easy to use and does not stick to gloves. You can safely adjust the position of the film and board until you are satisfied. What should I do if I don’t have enough samples for the ELISA experiment and can’t use all the wells? You can choose removable strips of sealing film, which can be torn off as much as you need, eliminating the tedious task of cutting the sealing film. Compared with using the entire membrane each time, it can effectively reduce the sample

Introduction to the Analytica Exhibition Analytica was founded in 1968 and is hosted by the Munich International Exhibition Group (MMI) in Germany. It is held every two years. Analytica2024 was attended by 882 exhibitors from 39 countries, and 26,573 visitors visited the exhibition. Analytica is an international event in the fields of world analysis, laboratory technology and biochemical technology. Aijin Biotechnology is setting sail to the sea and launching new products in 2024. Directly at the scene·Aijin debuts Aijin Biotech will exhibit high-quality domestic consumables at Analytica2024, including pipette tip series, deep well plate series, PCR series, column series, cell culture series, etc. Among them, the newly launched ELISA plate series products have an accuracy comparable to that of international brands and are extremely cost-effective, attracting many customers to come for communication and consultation. Directly visit the scene and communicate. Aijin Biotechnology always focuses on product research and development and quality assurance.

Cell experiment is an extremely important part of the life science discipline, which greatly promotes the development of the biomedical industry. It leads to a dazzling array of equipment, reagents, and consumables. There are many types of cell culture containers alone. This article will give an overview of cell culture products for your reference and study. Cell culture dish: It has a large opening and is easy to operate. It is suitable for use when extracting and cultivating primary cells, and is convenient for placing tissue fragments, etc., but it does not have a corresponding anti-contamination function, so you need to pay special attention to aseptic operation when conducting cell-related experiments. Cell culture bottle: It is the most suitable culture vessel for long-term cell culture, large-scale cell expansion and contamination prevention in the laboratory. The bottle mouth is small and equipped with a sealing cover or a breathable cover, which can effectively prevent contamination in the incubator and reduce the risk of contamination. However, the small bottle mouth also brings operational inconvenience. When performing cell passaging, it is best to use it with an extended tip or serological pipette, so that it is safer to reach the bottom of the bottle to absorb the culture medium.

The 21st China International Laboratory Medicine and Blood Transfusion Instruments and Reagents Expo (CACLP) concluded successfully on March 18, 2024 at the Chongqing International Expo Center. The conference lasted for three days and brought together 1,300+ companies from more than 20 countries and regions at home and abroad. Aijin Biotechnology appeared at booth N5-1016 and participated in this flagship professional exhibition and academic event that serves as an industry benchmark and leads the healthy development of the industry. At this exhibition, Aijin Biotech's various experimental consumable products were unveiled, including pipette tip series, deep well plate series, reservoir series, PCR series, column series, cell culture series, etc. With its rich product categories and high-quality product advantages, Aijin's booth attracted many customers to visit and consult.

Generally, when draining liquid, some liquid will remain inside the tip, which will cause certain errors in the experimental results. If this error is within an acceptable range, the experiment can be carried out using ordinary pipette tips. However, for experiments with high sensitivity requirements, or precious samples and reagents that are easy to remain, you need to choose low-adsorption tips. Aijin Bio's low-adsorption tips are made of imported medical-grade polypropylene (PP) material. The surface has been hydrophobically treated, which can minimize the residual surface tension liquid in the tip, in order to obtain more accurate experimental results and a higher recovery rate of precious samples.

The 2024 Munich International Analytical Biochemistry Expo (referred to as: Analytica 2024) will grandly open in Munich, Germany on April 9! At that time, Aijin Biotechnology will make a wonderful appearance with the latest experimental consumable products and solutions. We look forward to gathering with you in Munich, the charming capital of Europe, to discuss new products, new technologies, and new developments in the industry! Exhibition time: April 9-12, 2024, exhibition location: New International Exhibition Center in Munich, Germany, Aijin Booth: A3.226/6

The 21st China International Laboratory Medicine and Blood Transfusion Instruments and Reagents Expo (abbreviation: CACLP) will be held grandly at the Chongqing International Expo Center on March 16, 2024. With the theme of 'Cooperation and Sharing, Intelligence Leading the Future', this exhibition is a banner professional exhibition and academic event that serves as an industry benchmark and leads the healthy development of the industry. The conference is coming soon, and Aijin Biotech will appear with representative experimental consumables such as pipette tips, centrifuge tubes, deep well plates, and enzyme plate. We sincerely invite you to come to Shancheng to attend this experimental medicine and in vitro diagnostics feast.

PCR is a commonly used experimental technique in molecular biology laboratories, and almost every biological person uses it. But it is not easy to truly master PCR technology. Not to mention the experiment itself, even the consumables for PCR experiments hide a lot of knowledge. If you want to do your job well, you must first sharpen your tools. There are many types of PCR consumables. Today we will talk about their selection. How to choose between PCR single tubes, eight-tube tubes, and plates? The single tube can be flexibly selected according to the number of PCR experimental reactions. The eight-tube tube is suitable for medium and low-throughput experiments. One end of the cap has a protruding handheld end to reduce contamination when opening the cap. The eight-tube tube with cap can be opened and closed independently to prevent sample contamination. The eight-piece tube can also be used as a single tube by cutting it in the middle. PCR plate: suitable for high-throughput experiments. Alphanumeric markers help identify sample locations.

Cell culture requires cell consumables to achieve the conditions required for cell growth. Cell culture bottles and culture dishes are the two most commonly used cell culture consumables. So what is the difference between these two consumables? Cell culture bottles are the most suitable culture vessels for long-term cell culture, large-scale cell expansion and contamination prevention in the laboratory. The bottle mouth is small and the cells are not easily contaminated. Cells can be processed conveniently and safely to ensure the safety of the experimental process. The bottle mouth can be optional with a sealing cap/breathable cap. The sealing cover is often used for sealed culture to ensure its sealing, and it can be cultured in an ordinary constant temperature incubator. Breathable covers are often used for open culture to provide sterile gas exchange and reduce the risk of contamination. They are recommended for CO2 incubator culture, especially for experiments requiring long-term culture.