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Product Recommendation | How to choose an enzyme plate?
Views: 0 Author: Site Editor Publish Time: 2024-05-15 Origin: Site
Enzyme plate is an indispensable experimental tool in the Enzyme Linked Immunosorbent Assay (ELISA). It can be used to determine the purity, concentration and ratio of antigens and antibodies, labeled antibodies or antigens in immunological reactions.
01 How to choose transparent, white and black enzyme plates
The transparent plate is the most basic enzyme plate and can be used for detection of absorbed light and some basic color change reactions. White plates are mainly used to detect weaker light, such as chemiluminescence. The black plate itself has light absorption and is used for fluorescence detection of stronger light.
02 What is the basis for classifying high and medium binding enzyme plates?
The binding capacity of the enzyme plate is divided based on the binding ability of the empty plate to the protein. After the surface of the high-binding enzyme plate is treated, its protein binding capacity can reach 500~600ng IgG/cm2. The main binding protein is a protein with a molecular weight of 10,000 daltons, which is suitable for coating a large number of antigens and antibodies. The protein binding capacity of the medium-binding plate is 200-300 protein immunoglobulins per square centimeter. As a solid-phase carrier for macromolecular proteins with a molecular weight greater than 20,000 Daltons, its surface can reduce the number of non-specific adsorption sites and is suitable for experiments that require higher background signal or lower antigen and antibody concentrations.
03 The difference between enzyme plate and cell culture plate
ELISA plates have 96, 384, and 1536 holes, and are mainly used for enzyme-linked immunosorbent assays. They have high requirements for the consistency of pore diameters, uniformity of well bottoms, and transparency. The average light transmittance of Aijin microplate is >99% and cv<1%, which can reduce experimental errors and increase the accuracy of testing. Cell culture plates not only have 96 and 384 wells, but also 6, 12, 24, and 48 wells. After sterilization, they can be directly used for cell culture. The bottom of the plate is treated with TC and can be used for adherent culture of cells.
Therefore, the specific experimental scenarios of enzyme plate and cell culture plate are different, and the two cannot be mixed. When conducting experiments, we must clarify their respective characteristics and scope of application to ensure the accuracy and reliability of the experiment. Only by correctly selecting and using appropriate experimental equipment can we provide a strong guarantee for the success of the experiment, thereby promoting the continuous development and progress of scientific research!
04 Recommendation of experimental consumables-Aijin enzyme plate
Product introduction
Aijin microplates are made of high-quality imported polystyrene (PS) and have ultra-high sensitivity and specificity. They can accurately detect and analyze trace substances in biological samples, providing more reliable data support for scientific researchers. At the same time, its optimized reaction conditions and stable performance ensure the accuracy and repeatability of experimental results.
Design and Optimization
Two binding strengths are available: high (400~500ng/cm²) and medium (200~300ng/cm²); the adsorption effect of each hole is uniform and stable, and the error between holes is small; the transparent plate has high light transmittance, CV value <5%, and the measurement is more sensitive; the bottom is flat and uniform, reducing the There are few gaps between wells to ensure the accuracy of the experiment; the transparent/white/black plates are used for color development/chemiluminescence/fluorescence reactions respectively; there are clear numbers and letters on the edges of the holes to facilitate sample labeling; 1536 holes have an increased number of holes compared to 96/384 holes, and extremely low-volume sample loading is extremely cost-effective.
