Product Recommendation | How to choose PCR experimental consumables? 2023-12-04
PCR series products are mainly used as carriers for primers, Taq DNA polymerase, dNTPs, template nucleic acids, Mg, buffers, etc. that participate in the amplification reaction in the polymerase chain reaction (PCR). They are widely used in fields such as genetics, biochemistry, immunity, and medicine. They are not only used in basic research such as gene isolation, cloning, and nucleic acid sequence analysis, but can also be used in the diagnosis of diseases or anywhere where DNA and RNA are present. PCR series products are divided into single tubes, eight consecutive tubes, and plates. So how to choose different types of products? It is used when the sample amount is small. It can be used flexibly according to the number of samples. The tube body and tube cap are connected and are easy to be confused with microcentrifuge tubes, but they cannot be mixed. The walls of PCR tubes are thin, which helps ensure the speed and uniformity of heat conduction during PCR amplification, but they cannot withstand large centrifugal forces. The walls of microcentrifuge tubes are thicker, which meets the centrifugation requirements, but also results in slow and uneven heat transfer. When the sample volume increases, you can choose eight-strip tubes, which are generally available in transparent and white colors, and are suitable for ordinary PCR and qPCR respectively. Among them, the white eight-connected tube can effectively prevent signal interference, increase signal strength, and improve experimental efficiency.
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