In modern molecular biology research, efficient extraction and purification of nucleic acids is crucial. With the continuous advancement of technology, column membrane nucleic acid extraction has gradually become a commonly used method among scientific researchers. Below we will discuss in detail the working principle of the column membrane method, the specific structure of the nucleic acid purification column and its significant advantages in nucleic acid extraction, aiming to provide everyone with a multi-faceted understanding and practical reference. 01 Principle of nucleic acid extraction by column membrane method Column membrane method for nucleic acid extraction is widely used by scientific researchers because of its convenience, ease of use, and flexible throughput. The principle is to use a high-salt environment to destroy the structural membrane of water molecules on the surface of nucleic acids, causing the nucleic acids to be adsorbed on the spin column, while impurities such as proteins are precipitated by centrifugation, and the nucleic acids are separated and purified through the elution step. The main steps of the column membrane method include lysis, binding, washing and elution, and the nucleic acid purification column is used in the latter three steps. 02 Introduction to Nucleic Acid Purification Column Nucleic acid purification column is a tool used for extraction and purification of DNA and RNA. Read More

During the experiment, the use of centrifuge tubes often encounters various problems, which will directly affect the accuracy and safety of the experiment. For example, damaged centrifuge tubes can lead to experimental failure, unclear maximum speeds that the centrifuge tubes can withstand can cause safety hazards, and excessive liquid loading during cryopreservation can lead to tube explosion. Whether the material of the centrifuge tube is safe and whether it can be sterilized by high temperature and high pressure are also related to the reliability of the experiment. In order to solve these problems, Aijin centrifuge tubes have been completely upgraded to make your experimental process safer and more convenient. PRODUCT UPGRADE Five major upgrade points for centrifuge tubes Part 1 indicates the maximum centrifugal force and adopts a thickened tube wall design to ensure that the centrifuge tube will not deform under 12000xg centrifugal force, greatly improving safety. When used within the marked safe centrifugal force range, you no longer have to worry about centrifuge tube damage. Part 2 marks the maximum freezing line. The maximum freezing line is clearly marked on the pipe body. Read More

Plasmid extraction is one of the commonly used techniques in molecular biology experiments. Although the operation is simple, many people do not fully understand the principles behind it. Commonly used plasmid extraction kits on the market usually extract pure plasmid DNA by adding different solutions (such as solutions 1, 2, and 3), followed by washing and elution steps. However, the components of each solution and the specific role they play in the experiment are still unfamiliar to many people. Principle of Plasmid Extraction Experiment Principle In cells, there are a variety of biological macromolecules, including proteins, genomic DNA, plasmid DNA and RNA. The goal of plasmid extraction is to isolate pure plasmid DNA from these complex molecular mixtures without interference from other macromolecules. To achieve this, specific methods are needed to treat these different molecules individually. Genomic DNA and Plasmids Distinguish bacteria do not have a nucleus, only one Read More

Microbial isolation and purification are key processes to obtain single strains from mixed flora. This article takes the isolation of actinomycetes, fungi, and bacteria in soil as an example to introduce the wide application of plate separation method in the isolation and purification of microorganisms. Due to its simplicity and efficiency, the plate separation method can effectively obtain pure strains through different separation techniques and appropriate culture media, ensuring the accuracy of subsequent research and applications. 1. Dilution coating plate method The dilution coating plate method is a method in which bacterial liquid is diluted in a gradient and spread on the surface of a solid medium to eventually form a single bacterial colony. The principle is that in a bacterial solution with a high enough dilution, the microorganisms gathered together will be dispersed into single cells, thus forming a single colony on the surface of the culture medium. The specific steps are as follows: 1) Pour the plate to dissolve the beef extract peptone culture medium, Gao's No. 1 culture medium and PDA culture medium and cool them to 55~60°C. Add specific additives respectively and mix well before pouring. Read More

As a commonly used cell culture consumable, triangular culture shake flask has a unique conical design and is mainly used in cell and bacterial culture, media configuration, reagent storage, heating, cooling, etc. Advantages of PC triangular culture shake flask Triangular culture shake flask is a type of cell culture product based on Erlenmeyer flasks. Compared with glass Erlenmeyer flasks, PE has the following advantages: 1) Not easy to break; 2) The breathable cover is sealed and easy to take after heating; 3) It does not leak when used liquid to protect precious samples; 4) Clear scales and transparent bottle material make it easy to directly observe the situation inside the bottle during culture; 5) For large-capacity triangular shake flasks, there is an anti-slip mouth design on the bottle mouth, which is convenient for stable grip and prevents overweight and difficulty in handling after filling with liquid. The optimal working volume of the Erlenmeyer flask. When the Erlenmeyer flask is used for culture function, it will generally be in a shaking shaker environment. Too much may cause liquid to splash to the mouth of the bottle when shaking, increasing the risk of contamination and compressing the bottle. Read More

In the laboratory, do you sometimes want to talk about a certain consumable or equipment in English, but you get stuck? don’t worry! We have prepared for you an English guide to commonly used laboratory equipment. Whether you are a veteran in scientific research or a newbie, knowing the English names of these tools will make your laboratory life smoother and more interesting, and your scientific research will be happier! Basic Tools First, let’s start with some basic but crucial items. Every experiment usually starts with sample preparation, which requires extensive use of tubes and pipettes. Centrifuge tube: centrifugal tube Ep tube: Brand E tube Pipette: pipette Read More

In laboratory operations, choosing high-quality, convenient consumables can significantly improve work efficiency and the accuracy of experimental results. Aijin's column products are your ideal choice. Whether it is DNA extraction, gel recovery, or other related operations, we can provide you with all-round support to help the experiment run smoothly. Flexible application with various combinations Aijin 1.5mL and 2mL centrifuge tubes (long arm or without cap) products can be flexibly used with Aijin gel tubes, preparation tubes, and purification columns (with or without caps) for DNA extraction, gel recovery and other operations to meet different experimental needs. Convenience saves time and improves efficiency. Aijin's 1.5mL and 2mL boxed centrifuge tubes are not only convenient for storage, but the size also complies with SBS international standards and can be placed directly in the box for centrifugation. This design greatly simplifies the operation process and saves valuable experimental time and manpower. Ergonomic design, one-hand operation, Aijin micro-volume EP tube adopts snap-on tube cover design Read More

Bacterial endotoxin refers to a complex of lipopolysaccharide and protein on the cell wall of Gram-negative bacteria. Endotoxins are released after bacterial death or autolysis. Endotoxins entering the blood in large amounts can cause a fever reaction - 'pyrogenic reaction'. The bacterial endotoxin test method uses limulus reagent to detect or quantify bacterial endotoxins produced by Gram-negative bacteria to determine whether the endotoxin limits in the sample comply with regulations. The principle is that endotoxin activates factor C in the limulus reagent, causing the limulus reagent to undergo a series of enzyme-linked reactions, eventually forming a gel or activating the coagulase and adding a chromogenic group to detect bacterial endotoxin content. The main detection methods are divided into gel method and photometric method. 01 Qualitative and semi-quantitative gel method for endotoxin The gel method is mainly used for qualitative and semi-quantitative endotoxin. Although it is a standard method for endotoxin detection, the detection range is limited, and there are requirements for detection water and detection conditions. 02Turbidimetric method and chromogenic matrix method Photometric method Read More

Enzyme plate is an indispensable experimental tool in the Enzyme Linked Immunosorbent Assay (ELISA). It can be used to determine the purity, concentration and ratio of antigens and antibodies, labeled antibodies or antigens in immunological reactions. 01How to choose transparent, white and black enzyme plate HOW TO CHOOSE The transparent plate is the most basic enzyme plate and can be used for detection of absorbed light and some basic color change reactions. White plates are mainly used to detect weaker light, such as chemiluminescence. The black plate itself has light absorption and is used for fluorescence detection of stronger light. 02What is the basis for classifying high and medium binding enzyme plates? HOW TO DISTINGUISH The binding capacity of the enzyme plate is divided based on the binding ability of the empty plate and protein. After the surface of the high-binding enzyme plate is treated, its protein binding capacity can reach 500~600ng. Read More

In cell experiments, cells not adhering to the wall is a common and troubling problem. It often brings a lot of inconvenience to the experiment and affects the experiment and the accuracy of the results. When encountering a situation where cells are not adhering to the wall, we can start from the following aspects to investigate the reasons for the non-adherence and find corresponding solutions. ▲Adherent cell culture 01 Causes and solutions for cells not adhering REASONS AND SOLUTIONS Excessive trypsin digestion. Trypsin treatment for too long may cause damage to cell membrane proteins and affect cell adhesion; solution: adjust digestion time, shorten trypsin digestion time or reduce its concentration to reduce damage to cell membrane proteins. Microbial contamination Mycoplasma infection or other microbial contamination will cause cell dysfunction and affect the ability to adhere to the wall; solution: isolate the culture and detect mycoplasma. If mycoplasma contamination is found, the culture should be discarded. The pH value of the culture medium is too alkaline. Read More

In the laboratory, we often need to store samples such as cells and tissues in extremely low temperature environments for long-term preservation. However, when we take out samples that have been stored for a period of time from ultra-low temperature refrigerators or liquid nitrogen tanks, we often hear a sudden crackling sound of the cryogenic tube, which is alarming! This situation will not only result in the loss of experimental samples, but may also cause harm to experimental personnel. Therefore, it becomes crucial to understand why cryovials burst and how to avoid this. 01 Reasons for pipe explosion In experiments, we often need to freeze cells for long-term storage at a temperature of -130°C or lower. In a liquid nitrogen tank, the temperature of the gaseous layer ranges from -140°C to -180°C, but liquid nitrogen often infiltrates. The root cause of the cryopreservation tube bursting is that liquid nitrogen remains in the tube. When we take out the cryopreservation tube from the liquid nitrogen tank, the temperature inside the tube rises rapidly, causing the liquid nitrogen to vaporize rapidly and generate a large amount of nitrogen, thereby increasing the pressure inside the tube and eventually causing Read More

Recently, the global life science field has ushered in a unique industry event. Aijin Biotechnology, with its excellent lineup of domestically produced consumables, has appeared at the Shanghai CMEF and Russia's Analitika domestic and overseas dual exhibitions, which has injected new vitality into Aijin Biotechnology's brand influence and product competitiveness. At the same time, it has also written a significant stroke for the rise of domestic laboratory consumables on the global stage. CMEF & Analitika Domestic and Overseas Dual Exhibition Exhibition Introduction The 89th China International Medical Equipment (Spring) Expo (CMEF) will be held at the Shanghai National Convention and Exhibition Center from April 14 to 14, 2024. The exhibition has the theme of 'Innovative Technology, Leading the Future Intelligently', covering tens of thousands of full-department and full-industry chain product technologies and solutions such as in vitro diagnostics, medical consumables, and medical robots. The 2024 Russian International Laboratory Instruments and Equipment Exhibition (Referred to as Analitika Read More